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Technology Overview
VisiGen Biotechnologies, Inc.
is developing a revolutionary approach for DNA sequencing
that will enable rapid, inexpensive whole genome
sequencing.
Single-molecule
detection,
fluorescent
molecule
chemistry,
computational
biochemistry,
and genetic
engineering of
biomolecules,
are combined to
create this
novel sequencing
system.
VisiGen's
Sequencing Strategy:
-
Engineer
both polymerase and nucleotide triphosphates to act
together as direct molecular sensors
(nano-sequencing machines) of DNA base identity.
-
Monitor signals
from individual nano-sequencing machines (single
molecule detection).
-
Acquire data
during DNA strand synthesis (real-time detection).
-
Create massively
parallel arrays (microarrays) of these nanomachines.
-
Achieve a
sequencing rate of 1Mb/sec/machine.
-
Integrate a system of equipment, software, and
consumables for turn-key laboratory applications.
-
Ultimately, sequence an entire human genome for less
then $1000.
VisiGen's next generation sequencing technology
is distinguished from other leading developers:
-
Exploits the
natural process of DNA replication.
-
Enhances accuracy and
minimally impacts efficiency of DNA replication.
-
Requires minimal
sample amount that is prepared in a non-destructive
way.
-
Employs direct
analysis and eliminates the need for cloning or
amplification.
-
Eliminates
sequencing
reaction
processing and
electrophoresis.
-
Does not destroy the
original template material.
-
Does not produce an altered DNA polymer that may
negatively impact polymerase activity.
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